fingerprinting using matrix-assisted-laser desorption ionization-time of flight mass spectrometry. Proteins approximately 55 kDa and 60 kDa in size turned out to be CaHxk1 and Sir2 histone deacetylase respectively. Another sharp band having an apparent molecular mass of 80 kDa could be CaHXK1 Acts both as Repressor and an Activator of Genes Involved in Various Cellular Processes Apart from Regulating GlcNAc MedChemExpress JNJ-7777120 metabolic Genes Apart from their catalytic function, metabolic enzymes have acquired important regulatory functions controlling metabolism, stress resistance, growth and development in bacteria, yeasts, plants and animals. To elucidate the role of GlcNAc kinase in sugar signaling in Candida albicans, we used microarray for expression profile study. A comparative transcriptome analysis carried out with wild type and hxk1 mutant cells grown in 2% glucose at 30uC revealed differential regulation of several genes involved in various cellular processes. GO term analysis helped us to functionally categorize these genes Role of HXK1 in Candida albicans into over-represented groups viz., carbohydrate metabolic process, organic acid metabolic process, amino-acid metabolic process etc. Hyphal specific genes were also present in the differentially upregulated genes even though these genes did not fall in the over-represented functional category. The down regulated genes in the array data are mostly uncharacterized and could not be classified in to any functional category and hence omitted from the GO term analysis. Since in this array data-set, an important category comprised of the GlcNAc metabolic genes, we were interested to see the other Hxk1 regulated genes’ correlation with the GlcNAc mediated response. A 
transcriptome profile analysis between GlcNAc vs. glucose and GlcNAc vs. 18316589 Glycerol grown cells for wild type helped us to have an overview of role of Hxk1 in GlcNAc metabolism. To gain further insights into HXK1 mediated GlcNAc catabolic gene expression we performed q-RT PCR analysis of the genes like NGT1, NAG1 and DAC1 in presence of 5 mM glucose or GlcNAc that mimicked physiological conditions. Simultaneously to nullify catabolic repression we grew the wild type and mutant cells in glycerol and induced in glucose 2% or 5 mM Glucose or GlcNAc 5 mM. In hxk1 mutant the levels of derepression of these GlcNAc catabolic genes were comparable to the fold of induction in the wild type GlcNAc grown cells. While such a kind of result shows the Hxk1 dependence of these genes, it also brings into picture some additional means of regulation of their expression. This result also reveals another interesting fact that Hxk1 participates in maintaining the optimum levels of gene expression by exerting repressive effects on GlcNAc catabolic genes in presence of this amino sugar. Discussion Hxk1 Orchestrates Morphogenesis The response of organisms to environmental cues is often determined by the orchestration of different transcriptional circuits. We provide several lines of evidence to show that HXK1 mediated hyperfilamentation is quite unique and independent of the classical regulators. Moreover, an upregulation in the levels of HXK1 during filamentation induction probably hints at 15771452 it its role in maintaining an optimum level of expression of the filament specific genes through their repression. Recent studies indicate that morphological transitions are controlled at chromatin level through regulation of histone acetylases and methyltransferases. At thi