Ved, that LPA may be produced by many cell types such as fibroblasts andadipocytes, and it can be expressed in different tissues, including the brain, ovary, and kidney [9,11]. Also, it is known that LPA-1 is detected in many tumors, such as those in the lung, breast, stomach, kidney, and prostate [12]. It is well established that LPA signals various events through its G protein-coupled receptors (GPCRs), namely, LPA-1 to LPA-6 [9?4]. LPA-1, LPA-2, and LPA-3 share about 50?7 amino acid sequence identities 1676428 and form the Edg (Endothelial differentiation gene) family together with the GPCRs for sphingosine 1phosphate. The switching expression of LPA-1 receptors is found to be associated with prostate cancer development [1,13,15]. LPA4, LPA-5, and LPA-6 were classified as `non-Edg family’ LPA receptors and provide a new framework for understanding different LPA functions [13]. In normal tissues, LPA-1 is broadly expressed, whereas expression of LPA-2 and LPA-3 is more restricted [16]. The physiological role of LPA-4 has not beenLPA1 in Prostate Dysplastic Lesionsinvestigated [17] and LPA-5 was found to be highly expressed on cells associated with the immune system [18], and LPA-6 is a novel receptor implicated in human hair growth [13,14]. In the case of prostate cancer, LPA is reported to induce proliferation and survival of androgen-independent prostate cancer cells [9]. Prostate cancer is the second leading cause of male deaths in the majority of Western countries [11,19]. Information regarding the expression profile of LPA-1 in human biopsies is limited [11]. The rates of proliferation and apoptosis should be determined to have a better knowledge of the dynamism of the cell population in normal and pathological conditions and to establish the relationship with LPA-1 expression. On the other hand, angiogenesis is a critical feature of many diseases, including cancers and their precursors [20,21]. Angiogenesis is defined as the process leading to the formation of new blood vessels and is essential for normal growth and development [22]. Recently, LPA was demonstrated to promote ovarian cancer growth by inducing angiogenic factors [10], but this relation in prostate dysplastic lesions needs further investigation. A useful experimental model for human prostate cancer is the rat [23?5], and morphological similarities Bexagliflozin web between human PIN and dysplastic changes experimentally promoted in HDAC-IN-3 rodent prostate have been reported [26]. Our group designed an experimental model based on the administration of low doses of cadmium chloride [6?,27]. This model induces higher incidence of prostate carcinogenesis in Sprague-Dawley rats in a manner similar to those in humans. In the present study, we estimated immunoexpression and quantification of LPA-1 
in epithelial cells. Cell proliferation was determined by the quantification of proliferative cell nuclear antigen (PCNA) and the miniature chromosome maintenance (MCM7). The apoptosis was quantified using Bcl-2, ubiquitin, and p53 and by measuring the ratio of apoptotic nuclei to the total nuclei of epithelial cells using the TUNEL assay. The angiogenesis was observed by quantification of Von Willebrand factor (Factor VIII). The aims of this study are as follows: a) to determine the LPA-1 immunoexpression in preneoplastic lesions induced with cadmium chloride; b) the evaluation of cell proliferation, apoptosis, and angiogenesis markers in these lesions; and c) to determine the correlation between LPA-1 immunoe.Ved, that LPA may be produced by many cell types such as fibroblasts andadipocytes, and it can be expressed in different tissues, including the brain, ovary, and kidney [9,11]. Also, it is known that LPA-1 is detected in many tumors, such as those in the lung, breast, stomach, kidney, and prostate [12]. It is 
well established that LPA signals various events through its G protein-coupled receptors (GPCRs), namely, LPA-1 to LPA-6 [9?4]. LPA-1, LPA-2, and LPA-3 share about 50?7 amino acid sequence identities 1676428 and form the Edg (Endothelial differentiation gene) family together with the GPCRs for sphingosine 1phosphate. The switching expression of LPA-1 receptors is found to be associated with prostate cancer development [1,13,15]. LPA4, LPA-5, and LPA-6 were classified as `non-Edg family’ LPA receptors and provide a new framework for understanding different LPA functions [13]. In normal tissues, LPA-1 is broadly expressed, whereas expression of LPA-2 and LPA-3 is more restricted [16]. The physiological role of LPA-4 has not beenLPA1 in Prostate Dysplastic Lesionsinvestigated [17] and LPA-5 was found to be highly expressed on cells associated with the immune system [18], and LPA-6 is a novel receptor implicated in human hair growth [13,14]. In the case of prostate cancer, LPA is reported to induce proliferation and survival of androgen-independent prostate cancer cells [9]. Prostate cancer is the second leading cause of male deaths in the majority of Western countries [11,19]. Information regarding the expression profile of LPA-1 in human biopsies is limited [11]. The rates of proliferation and apoptosis should be determined to have a better knowledge of the dynamism of the cell population in normal and pathological conditions and to establish the relationship with LPA-1 expression. On the other hand, angiogenesis is a critical feature of many diseases, including cancers and their precursors [20,21]. Angiogenesis is defined as the process leading to the formation of new blood vessels and is essential for normal growth and development [22]. Recently, LPA was demonstrated to promote ovarian cancer growth by inducing angiogenic factors [10], but this relation in prostate dysplastic lesions needs further investigation. A useful experimental model for human prostate cancer is the rat [23?5], and morphological similarities between human PIN and dysplastic changes experimentally promoted in rodent prostate have been reported [26]. Our group designed an experimental model based on the administration of low doses of cadmium chloride [6?,27]. This model induces higher incidence of prostate carcinogenesis in Sprague-Dawley rats in a manner similar to those in humans. In the present study, we estimated immunoexpression and quantification of LPA-1 in epithelial cells. Cell proliferation was determined by the quantification of proliferative cell nuclear antigen (PCNA) and the miniature chromosome maintenance (MCM7). The apoptosis was quantified using Bcl-2, ubiquitin, and p53 and by measuring the ratio of apoptotic nuclei to the total nuclei of epithelial cells using the TUNEL assay. The angiogenesis was observed by quantification of Von Willebrand factor (Factor VIII). The aims of this study are as follows: a) to determine the LPA-1 immunoexpression in preneoplastic lesions induced with cadmium chloride; b) the evaluation of cell proliferation, apoptosis, and angiogenesis markers in these lesions; and c) to determine the correlation between LPA-1 immunoe.