proteins were purified over Ni2+-NTA agarose under denaturing conditions. The proteins were then greatly diluted and refolded in the presence of glutathione redox pair and were further purified by anion exchange chromatography. P12 and P41 polyclonal rabbit sera and P12 mouse monoclonal antibodies were produced at the Walter & Eliza Hall Institute Monoclonal Antibody Facility. Immunofluorescence microscopy Late schizonts and merozoites were fixed with 4% paraformaldehyde/0.0075% glutaraldehyde as described previously. The 
cells were labelled with mouse and rabbit antibodies specific to MSP1, P12, and P41 as indicated and corresponding secondary antibodies Alexa Fluor 488 goat antirabbit IgG and Alexa Fluor 568 goat anti-mouse IgG. The images were captured using Zeiss AxioObserver Z1 fluorescence microscope and analysed with ImageJ software. Mammalian expression and avidity-based extracellular interaction screening To express P12 and P41 in a mammalian expression system, the regions of the genes encoding the predicted ectodomain fragments were chemically synthesized so that their codons were optimized for expression in human cells. In addition, any potential N-linked glycosylation sequons were mutated to prevent inappropriate glycosylation and an exogenous signal peptide was used and cloned N-terminal to a rat Cd4 domain 3 and 4 tag using flanking NotI and AscI restriction sites as described. Proteins were produced as secreted 66His tagged recombinant proteins using HEK293E cells and purified using Ni2+-NTA resin, essentially as described. AVEXIS assays were performed as described. Western blot analysis of recombinant proteins and parasites Recombinant E. coli produced P12 and P41 proteins were fractionated by SDS-PAGE under non-reducing and reducing Biochemical and Functional Analysis of P12 and P41 Size exclusion and surface plasmon resonance Binding of recombinant P12 and 41 to form a heterodimer was examined by column shift assay. Equimolar amounts of recP12-Cd4d3/4-6H and recP41-Cd4d3/4-6H were co-incubated for 1 hr at 37uC in phosphate buffer. Following incubation samples were centrifuged for 10 minutes at 180006 g at 4uC prior to filtration through 0.2 mm Acrodisc 13 mm Syringe Filters. This material underwent gel filtration chromatography using a Superdex 200 10/300 GL column in PBS on an AKTA Purifier. recP12-Cd4d3/46H and recP41-Cd4d3/4-6H were also subjected to gel filtration independently to determine their individual retention volumes as a point of comparison with the heterodimer. Biophysical binding analysis was performed by surface plasmon resonance using a T100 instrument. Briefly, proteins were captured on streptavidin-coated sensor chips by a C-terminal enzymatically biotinylatable tag ��bio��as described. Approximately 150RU of biotinylated rat Cd4d3/4 was captured in the flow cell PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/22205151 used as a MedChemExpress BIBW2992 reference and approximate molar equivalents of recP12-Cd4d3/4bio or recP41-Cd4d3/4-bio immobilised in the other flow cell. Purified recP12-Cd4d3/4-6H or recP41-Cd4d3/4-6H proteins were resolved by gel filtration just prior to use in SPR experiments to remove small amounts of protein aggregates which are known to influence kinetic binding measurements. Increasing concentrations of purified proteins were injected at high flow rates to minimise rebinding effects for kinetic studies or at 10 mL/ min for equilibrium analysis. Both kinetic and equilibrium binding data were analysed in the manufacturer’s evaluation software. Equilibrium bi