Tubulin was utilized as a handle to demonstrate the presence of equivalent quantity of proteins in each and every lane and was immunoblotted using anti-Tubulin antibody
(C) In cells expressing GFP-Ecm33 from the chromosomally built-in gene, GST-ubiquitin was expressed from the harboring plasmid at 27uC. GST-tagged ubiquitin was pulled down by glutathione beads, washed extensively, subjected…