Ese disturbances in which the largest spatial clusters of sites were identified, match some of them with key events that occurred in Rwanda, and discuss what we learn from each matched and unmatched event. This serves as a test of how well our system identifies key events in Rwanda and to highlight the next steps towards the goal of creating an effective emergency event identification system with mobile phone data. As discussed earlier, although our aim is to identify emergency events, non-emergency events might also cause behavioral changes. It is important to understand the particular behavioral signatures of many different events, in order to effectively identify and differentiate those that are emergencies. In this regard, our system PXD101MedChemExpress PXD101 identified several kinds of events, from hazards to holidays, all of which we now discuss. Violence against civilians–September 16, 2005 (Fig. I in S1 Supporting Information). Our system identified a spatial cluster of four sites with higher than usual call volume and movement frequency on September 17, 2005. These sites are located in the vicinity of the city of Goma, along the Rwanda-DRC border and near the Rwanda-Uganda border. We find no event, emergency or otherwise that occurred on September 17, 2005. However, a violent event, reported by ACLED, occurred in the same area on September 16, 2005. The distance between the event’s reported location (latitude -1.67, longitude 29.22) and the centroid of the closest site in the spatial cluster is 1.7 km. Radio France reported that this event involved five armed attacks in which 1 person was killed, 7 others were wounded, and was part of the general trend of violence against civilians. Upon close consideration, the one day lag between the event and the behavioral changes is reasonable. It is likely that the attacks were directly Q-VD-OPh biological activity witnessed by a relatively small proportion of the local population who then communicated this information to others and the information propagated from there. As such, it could take a full day until a large proportion of the local population heard about and reacted to the event. From this case we gain a two key insights. First, our system can identify behavioral anomalies that are reasonable matches to emergency events. Second, information propagation might take time to reach a large portion of an affected population and thus behavioral anomalies that are identifiable at the population level might occur well after an event. Violence against civilians–January 15, 2006 (Fig. J in S1 Supporting Information). We identify 16 sites (eight in the Kigali area) with lower than usual call volume on January 15, 2006. Eight of these sites also had lower than usual movement frequency. A violent event was recorded by ACLED in Kigali on the same day. The distance between the event’s reported location (latitude -1.96, longitude 30.04) and the centroid of the closest site with unusual call volume is 3.8 km. Reporters Without Borders and Amnesty International report that four armed men invaded and ransacked the home of Bonaventure Bizumuremyi, the editor of the independent fortnightly Rwandan newspaper, Umuco. Mr. Bizumuremyi was the target of intimidation and harassment, demanding that he stop publishing articles criticizing the ruling Rwandan Patriotic Front (RPF). The armed forces of the RPF forced many independent journalists and human rights activists to leave Rwanda during that same general time period by intimidation,PLOS ONE | DOI:10.1371/.Ese disturbances in which the largest spatial clusters of sites were identified, match some of them with key events that occurred in Rwanda, and discuss what we learn from each matched and unmatched event. This serves as a test of how well our system identifies key events in Rwanda and to highlight the next steps towards the goal of creating an effective emergency event identification system with mobile phone data. As discussed earlier, although our aim is to identify emergency events, non-emergency events might also cause behavioral changes. It is important to understand the particular behavioral signatures of many different events, in order to effectively identify and differentiate those that are emergencies. In this regard, our system identified several kinds of events, from hazards to holidays, all of which we now discuss. Violence against civilians–September 16, 2005 (Fig. I in S1 Supporting Information). Our system identified a spatial cluster of four sites with higher than usual call volume and movement frequency on September 17, 2005. These sites are located in the vicinity of the city of Goma, along the Rwanda-DRC border and near the Rwanda-Uganda border. We find no event, emergency or otherwise that occurred on September 17, 2005. However, a violent event, reported by ACLED, occurred in the same area on September 16, 2005. The distance between the event’s reported location (latitude -1.67, longitude 29.22) and the centroid of the closest site in the spatial cluster is 1.7 km. Radio France reported that this event involved five armed attacks in which 1 person was killed, 7 others were wounded, and was part of the general trend of violence against civilians. Upon close consideration, the one day lag between the event and the behavioral changes is reasonable. It is likely that the attacks were directly witnessed by a relatively small proportion of the local population who then communicated this information to others and the information propagated from there. As such, it could take a full day until a large proportion of the local population heard about and reacted to the event. From this case we gain a two key insights. First, our system can identify behavioral anomalies that are reasonable matches to emergency events. Second, information propagation might take time to reach a large portion of an affected population and thus behavioral anomalies that are identifiable at the population level might occur well after an event. Violence against civilians–January 15, 2006 (Fig. J in S1 Supporting Information). We identify 16 sites (eight in the Kigali area) with lower than usual call volume on January 15, 2006. Eight of these sites also had lower than usual movement frequency. A violent event was recorded by ACLED in Kigali on the same day. The distance between the event’s reported location (latitude -1.96, longitude 30.04) and the centroid of the closest site with unusual call volume is 3.8 km. Reporters Without Borders and Amnesty International report that four armed men invaded and ransacked the home of Bonaventure Bizumuremyi, the editor of the independent fortnightly Rwandan newspaper, Umuco. Mr. Bizumuremyi was the target of intimidation and harassment, demanding that he stop publishing articles criticizing the ruling Rwandan Patriotic Front (RPF). The armed forces of the RPF forced many independent journalists and human rights activists to leave Rwanda during that same general time period by intimidation,PLOS ONE | DOI:10.1371/.