Product Name :
IHC enzyme antigen retrieval reagent
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Clear slightly viscous solution.
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IHC enzyme antigen retrieval reagent is designed to unmask immunoreactive sites altered by fixation and/or the embedding process.The most commonly used tissue fixatives are aldehydes, such as formalin, which work by protein cross-linking.{{1418628-81-5} web|{1418628-81-5} Protocol|{1418628-81-5} Description|{1418628-81-5} custom synthesis} Proper fixation of a specimen prevents antigen from being washed out and is a critical aspect of successful IHC staining. Cross-linking can mask antigens, preventing primary antibodies from binding, thus yielding weak staining or false negative results. Immunoglobulins are especially susceptible to formalin fixation. Treatment with a proteolytic enzyme is required to digest excess aldehyde linkages and expose the antigen. The IHC enzyme antigen retrieval reagent is a proteolytic enzyme solution that results in superior staining over traditionally used enzyme pretreatments, such as pepsin and trypsin.{{1585973-65-4} site|{1585973-65-4} Protocol|{1585973-65-4} In Vivo|{1585973-65-4} manufacturer} Moreover, the IHC enzyme antigen retrieval reagent requires only a brief incubation at room temperature.PMID:25905171 Performing antigen recovery with the IHC enzyme antigen retrieval reagent will allow you to dilute the primary antibody 5 to 10 fold further and achieve optimal staining.
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| Appearance Clear slightly viscous solution. | Formulation Liquid. Enzyme antigen retrieval reagent. | Technical Info / Product Notes Interpretation of the results will be the sole responsibility of the user.