Ed to the induction of apoptosis. Cultured HeLa cells treated with MECS (5?00 g/mL) exhibited morphological features typical of apoptosis, which was consistent with the dose- and time-dependent DNA fragmentation, as determined by agarose gel electrophoresis (Figure 1). Several caspases play important roles in the regulation of apoptosis. They are grouped broadly as initiator or effector caspases according to the roles they play in inducing the apoptotic system. The initiator caspases include caspase-1, -8, and -9. Caspase-8 and -9 are typically activated by two alternative pathways. The firstHung et al. Biological Research 2014, 47:20 http://www.biolres.com/content/47/1/Page 3 ofTable 2 Cytotoxic activity of extracts from C. sappanExtracts HL-60 MeOH ex. EtOH ex. Water ex. CamptothecinaIC50 value (g/mL)a HeLa 26.5 ?3.2 39.2 ?2.0 37.8 ?3.6 3.4 ?0.2 MCF7 37.7 ?3.1 > 100 > 100 4.5 ?0.3 LLC > 100 25.1 ?3.8 > 100 5.1 ?0.7 HepG2 65.1 ?3.5 R848 price PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27532042 > 30 78.6 ?4.3 6.2 ?0.4 KPL4 > 100 > 100 > 100 14.5 ?1.7 HT-29 > 100 > 100 > 100 10.7 ?0.8 KB 76.7 ?4.8 > 100 > 100 4.5 ?0.4 40.7 ?5.8 68.5 ?5.1 >b6.2 ?0.The inhibitory effects are represented as giving 50 inhibition (IC50) relative to the vehicle control. These data represent the average values of three repeated experiments (mean ?S.D.). b Positive control.involves cell death receptor-mediated apoptosis via caspase-8 and is characterized by binding of cell death ligands and cell death receptors and subsequent activation of caspase-8 and -3. The second pathway involves mitochondria-mediated apoptosis via caspase-9. In both pathways, caspase-3 activation plays a central role in the initiation of apoptosis [25]. MECS concentrations of 5?00 g/mL induced caspase-3 activation in dose- and time-dependent manners (Figure 2). Many models of apoptosis include loss of mitochondrial transmembrane potential mediated by opening of the mega-channel, which precedes caspase activation. Caspase-3 activation is required for several typical hallmarks of apoptosis and is indispensable for apoptotic chromatin condensation and DNA fragmentation.investigation to determine its bioactive components is currently in progress.MethodsPlant materialThe heartwood of Caesalpinia sappan L. was BUdR cancer collected in An Giang province, Vietnam, in September 2010. Professor Tran Cong Luan at Vietnam National Institute of Medicinal Material botanically authenticated the plant. A voucher specimen number TCL-00120 describing the plant was deposited at the institute.Extract preparationConclusion This study for the first time determined that MECS effectively inhibit the proliferation of HeLa cells by mechanism involved the induction of apoptosis. According to these results, it is suggested that the methanol extract of Vietnamese C. sappan may be a considerable source for the development of anti-cancer drugs. FurtherThe extracts were prepared according to World Health Organization protocol (CG-1983) with a slight modification. The plant was dried and powdered, 50 g powder was extracted with 500 mL of methanol, ethanol or distilled water (3 times) for 3 hours under reflux. After extractions, the extracts were combined and filtered with Whatman filter paper No.1, and then were concentrated in vacuo to dryness. All extracts were stored at -4 until use.Figure 1 Induction of the DNA fragmentation in HeLa cells in vitro. HeLa cells were treated with MECS for 24 h (50, 20, 10 g/mL), and for 36 and 48 h (50, 20 g/mL). Total genomic DNA was extracted and resolved o.Ed to the induction of apoptosis. Cultured HeLa cells treated with MECS (5?00 g/mL) exhibited morphological features typical of apoptosis, which was consistent with the dose- and time-dependent DNA fragmentation, as determined by agarose gel electrophoresis (Figure 1). Several caspases play important roles in the regulation of apoptosis. They are grouped broadly as initiator or effector caspases according to the roles they play in inducing the apoptotic system. The initiator caspases include caspase-1, -8, and -9. Caspase-8 and -9 are typically activated by two alternative pathways. The firstHung et al. Biological Research 2014, 47:20 http://www.biolres.com/content/47/1/Page 3 ofTable 2 Cytotoxic activity of extracts from C. sappanExtracts HL-60 MeOH ex. EtOH ex. Water ex. CamptothecinaIC50 value (g/mL)a HeLa 26.5 ?3.2 39.2 ?2.0 37.8 ?3.6 3.4 ?0.2 MCF7 37.7 ?3.1 > 100 > 100 4.5 ?0.3 LLC > 100 25.1 ?3.8 > 100 5.1 ?0.7 HepG2 65.1 ?3.5 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27532042 > 30 78.6 ?4.3 6.2 ?0.4 KPL4 > 100 > 100 > 100 14.5 ?1.7 HT-29 > 100 > 100 > 100 10.7 ?0.8 KB 76.7 ?4.8 > 100 > 100 4.5 ?0.4 40.7 ?5.8 68.5 ?5.1 >b6.2 ?0.The inhibitory effects are represented as giving 50 inhibition (IC50) relative to the vehicle control. These data represent the average values of three repeated experiments (mean ?S.D.). b Positive control.involves cell death receptor-mediated apoptosis via caspase-8 and is characterized by binding of cell death ligands and cell death receptors and subsequent activation of caspase-8 and -3. The second pathway involves mitochondria-mediated apoptosis via caspase-9. In both pathways, caspase-3 activation plays a central role in the initiation of apoptosis [25]. MECS concentrations of 5?00 g/mL induced caspase-3 activation in dose- and time-dependent manners (Figure 2). Many models of apoptosis include loss of mitochondrial transmembrane potential mediated by opening of the mega-channel, which precedes caspase activation. Caspase-3 activation is required for several typical hallmarks of apoptosis and is indispensable for apoptotic chromatin condensation and DNA fragmentation.investigation to determine its bioactive components is currently in progress.MethodsPlant materialThe heartwood of Caesalpinia sappan L. was collected in An Giang province, Vietnam, in September 2010. Professor Tran Cong Luan at Vietnam National Institute of Medicinal Material botanically authenticated the plant. A voucher specimen number TCL-00120 describing the plant was deposited at the institute.Extract preparationConclusion This study for the first time determined that MECS effectively inhibit the proliferation of HeLa cells by mechanism involved the induction of apoptosis. According to these results, it is suggested that the methanol extract of Vietnamese C. sappan may be a considerable source for the development of anti-cancer drugs. FurtherThe extracts were prepared according to World Health Organization protocol (CG-1983) with a slight modification. The plant was dried and powdered, 50 g powder was extracted with 500 mL of methanol, ethanol or distilled water (3 times) for 3 hours under reflux. After extractions, the extracts were combined and filtered with Whatman filter paper No.1, and then were concentrated in vacuo to dryness. All extracts were stored at -4 until use.Figure 1 Induction of the DNA fragmentation in HeLa cells in vitro. HeLa cells were treated with MECS for 24 h (50, 20, 10 g/mL), and for 36 and 48 h (50, 20 g/mL). Total genomic DNA was extracted and resolved o.